Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Binding Sites / genetics
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Catalytic Domain / genetics
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DNA / genetics
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DNA Breaks, Double-Stranded*
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DNA Repair
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism
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Deoxyribonucleases, Type II Site-Specific / chemistry
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Deoxyribonucleases, Type II Site-Specific / genetics*
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Deoxyribonucleases, Type II Site-Specific / metabolism*
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Gene Targeting*
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Genetic Engineering*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription, Genetic
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Transcriptional Activation
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Xanthomonas
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Xanthomonas campestris
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Zinc Fingers / genetics
Substances
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DNA-Binding Proteins
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Recombinant Fusion Proteins
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Transcription Factors
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DNA
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endodeoxyribonuclease FokI
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Deoxyribonucleases, Type II Site-Specific