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Page 1
Glycoconjugates on the surface of epididymal spermatozoa in a marsupial, the brushtail possum, Trichosurus vulpecula.
Cooper NJ, McClean RV, Leigh CM, Breed WG. Cooper NJ, et al. Among authors: mcclean rv. Reproduction. 2001 Jul;122(1):165-76. doi: 10.1530/rep.0.1220165. Reproduction. 2001. PMID: 11425341
Variation in localization and distribution of saccharides on the sperm surface of a marsupial, the brushtail possum, Trichosurus vulpecula, was compared between spermatozoa from the caput and cauda epididymides. ...In general, the findings indicate similar glycosyla …
Variation in localization and distribution of saccharides on the sperm surface of a marsupial, the brushtail possum, Trichosurus vulp …
Sperm membrane fatty acid composition in the Eastern grey kangaroo (Macropus giganteus), koala (Phascolarctos cinereus), and common wombat (Vombatus ursinus) and its relationship to cold shock injury and cryopreservation success.
Miller RR Jr, Sheffer CJ, Cornett CL, McClean R, MacCallum C, Johnston SD. Miller RR Jr, et al. Among authors: mcclean r. Cryobiology. 2004 Oct;49(2):137-48. doi: 10.1016/j.cryobiol.2004.06.002. Cryobiology. 2004. PMID: 15351685
Marsupial spermatozoa tolerate cold shock well, but differ in cryopreservation tolerance. ...The highest proportions of DHA (22:6, n-3), the predominant membrane fatty acid in cauda epididymidal spermatozoa, were found in wombat and koala spermatozoa. …
Marsupial spermatozoa tolerate cold shock well, but differ in cryopreservation tolerance. ...The highest proportions of DHA (2 …
Ultrastructural observations of cryoinjury in kangaroo spermatozoa.
McClean RV, Holt WV, Johnston SD. McClean RV, et al. Cryobiology. 2007 Jun;54(3):271-80. doi: 10.1016/j.cryobiol.2007.03.004. Epub 2007 Mar 24. Cryobiology. 2007. PMID: 17462621
Macropod spermatozoa have proven difficult to cryopreserve such that empirical studies using high concentrations of glycerol and/or DSMO have resulted in only 10% post-thaw motility. We examined the ultrastructure and freeze-fracture of caput and cauda epididymal macropod …
Macropod spermatozoa have proven difficult to cryopreserve such that empirical studies using high concentrations of glycerol and/or D …
Cryopreservation of kangaroo spermatozoa using alternative approaches that reduce cytotoxic exposure to glycerol.
McClean R, Zee YP, Holt WV, Johnston SD. McClean R, et al. Cryobiology. 2008 Dec;57(3):304-7. doi: 10.1016/j.cryobiol.2008.08.007. Epub 2008 Sep 11. Cryobiology. 2008. PMID: 18822280
Immediate in-straw post-thaw dilution of 20% glycerol and cryopreservation of spermatozoa in 20% DMSO produced no significant improvement in post-thaw viability of kangaroo spermatozoa. Spermatozoa frozen in 20% DMA showed post-thaw motility and plasma membra …
Immediate in-straw post-thaw dilution of 20% glycerol and cryopreservation of spermatozoa in 20% DMSO produced no significant improve …
The effect of cryoprotectant on kangaroo sperm ultrastructure and mitochondrial function.
McClean R, Holt WV, Zee YP, Lisle A, Johnston SD. McClean R, et al. Cryobiology. 2008 Dec;57(3):297-303. doi: 10.1016/j.cryobiol.2008.08.006. Epub 2008 Sep 6. Cryobiology. 2008. PMID: 18805409
As expected, incubation of spermatozoa in 20% glycerol for 10 min resulted in a significant reduction in motility, PMI and ultrastructural integrity. ...Future research should be directed at investigating strategies that reduce exposure of spermatozoa to glycerol du …
As expected, incubation of spermatozoa in 20% glycerol for 10 min resulted in a significant reduction in motility, PMI and ultrastruc …
Ultrastructure, osmotic tolerance, glycerol toxicity and cryopreservation of caput and cauda epididymidal kangaroo spermatozoa.
McClean R, MacCallum C, Blyde D, Holt WV, Johnston SD. McClean R, et al. Reprod Fertil Dev. 2006;18(4):469-76. doi: 10.1071/rd05148. Reprod Fertil Dev. 2006. PMID: 16737640

Plasma membranes of caput epididymidal spermatozoa were more tolerant of hypo-osmotic media than were cauda epididymidal spermatozoa (P < 0.05). ...At both temperatures, caput epididymidal spermatozoa showed less plasma membrane damage compared with cauda

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Plasma membranes of caput epididymidal spermatozoa were more tolerant of hypo-osmotic media than were cauda epididymidal spermatoz …
An investigation into the similarities and differences governing the cryopreservation success of koala (Phascolarctos cinereus: goldfuss) and common wombat (Vombatus ursinus: shaw) spermatozoa.
Johnston SD, MacCallum C, Blyde D, McClean R, Lisle A, Holt WV. Johnston SD, et al. Among authors: mcclean r. Cryobiology. 2006 Oct;53(2):218-28. doi: 10.1016/j.cryobiol.2006.06.001. Epub 2006 Aug 2. Cryobiology. 2006. PMID: 16889764

Wombat spermatozoa showed better (P < 0.01) post-thaw survival (% motile, % intact plasma membranes, % decondensed sperm heads) than koala spermatozoa. When exposed to media of varying osmolality, koala spermatozoa were less tolerant (% intact plasma membr

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Wombat spermatozoa showed better (P < 0.01) post-thaw survival (% motile, % intact plasma membranes, % decondensed sperm heads) th

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Actin localisation and the effect of cytochalasin D on the osmotic tolerance of cauda epididymidal kangaroo spermatozoa.
McClean R, MacCallum C, Blyde D, Holt W, Johnston S. McClean R, et al. Cryo Letters. 2006 Jul-Aug;27(4):253-60. Cryo Letters. 2006. PMID: 16990953
In the first study, the distribution of G and F actin within Eastern Grey Kangaroo (EGK, Macropus giganteus) cauda epididymidal spermatozoa was successfully detected using DNAse-FITC and a monoclonal F-actin antibody (ab205, Abcam), respectively. ...To investigate whether …
In the first study, the distribution of G and F actin within Eastern Grey Kangaroo (EGK, Macropus giganteus) cauda epididymidal spermatoz …