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Comparison of bioactive substances in novel-developed freeze-dried platelet-rich plasma (PRP) and activated normal PRP, and investigation of bioactive substance levels after long-term storage.
Nakajima R, Saita Y, Kobayashi Y, Wakayama T, Uchino S, Momoi Y, Yamamoto N, Ishijima M. Nakajima R, et al. Among authors: wakayama t. Regen Ther. 2024 Mar 27;27:200-206. doi: 10.1016/j.reth.2024.03.021. eCollection 2024 Dec. Regen Ther. 2024. PMID: 38571893 Free PMC article.
However, whether VFD contains sufficient levels of bioactive substances (BS) remains unknown and retains the same levels of BS during long-term storage. In this study, we examined whether VFD contains sufficient amounts of BS and whether they retain these BS levels during …
However, whether VFD contains sufficient levels of bioactive substances (BS) remains unknown and retains the same levels of BS during long- …
Successful Production of Offspring Derived from Phospholipase C Zeta-Deficient Sperm by Additional Artificial Activation.
Hirose N, Kikuchi Y, Kageyama A, Sugita H, Sakurai M, Kawata Y, Terakawa J, Wakayama T, Ito J, Kashiwazaki N. Hirose N, et al. Among authors: wakayama t. Life (Basel). 2023 Apr 10;13(4):980. doi: 10.3390/life13040980. Life (Basel). 2023. PMID: 37109509 Free PMC article.
In this study, we examined whether oocytes injected with a Plcz1(-/-) sperm can develop to term by additional artificial activation. In oocytes injected a Plcz1(-/-) sperm and Plcz1(-/-) and eCS (another candidate of the sperm factor) double knockout sperm (Plcz1(-/-)eCS(- …
In this study, we examined whether oocytes injected with a Plcz1(-/-) sperm can develop to term by additional artificial activation. …
Production of offspring from vacuum-dried mouse spermatozoa and assessing the effect of drying conditions on sperm DNA and embryo development.
Ushigome N, Wakayama S, Yamaji K, Ito D, Ooga M, Wakayama T. Ushigome N, et al. Among authors: wakayama t. J Reprod Dev. 2022 Aug 1;68(4):262-270. doi: 10.1262/jrd.2022-048. Epub 2022 Jun 7. J Reprod Dev. 2022. PMID: 35676029 Free PMC article.
The volume, temperature, and viscosity of the medium were optimized to improve the birth rate. The results obtained from a comet assay indicated that decreasing the drying rate reduced the extent of DNA damage in VD sperm. ...These results indicate that the lower birth …
The volume, temperature, and viscosity of the medium were optimized to improve the birth rate. The results obtained from a comet assa …
Long-term follow-up of patients with ATL after autologous stem cell transplantation.
Utsunomiya A, Tokunaga M, Nakano N, Fujiwara H, Miyamoto T, Ogata M, Miyazaki Y, Ishitsuka K, Sakaida E, Taji H, Wakayama T, Ichinohe T, Fukuda T, Atsuta Y, Kato K, Yoshimitsu M. Utsunomiya A, et al. Among authors: wakayama t. Bone Marrow Transplant. 2022 Feb;57(2):323-325. doi: 10.1038/s41409-021-01412-9. Epub 2022 Jan 23. Bone Marrow Transplant. 2022. PMID: 35066568 Free PMC article. No abstract available.
Mouse in vivo-derived late 2-cell embryos have higher developmental competence after high osmolality vitrification and -80°C preservation than IVF or ICSI embryos.
Hayashi E, Wakayama S, Ito D, Hasegawa A, Mochida K, Ooga M, Ogura A, Wakayama T. Hayashi E, et al. Among authors: wakayama t. J Reprod Dev. 2022 Apr 1;68(2):118-124. doi: 10.1262/jrd.2021-115. Epub 2022 Jan 4. J Reprod Dev. 2022. PMID: 34980785 Free PMC article.
Further, IVF embryos had a lower survival rate after warming, but a similar rate to the in vivo embryos to full-term development. This result was confirmed by simultaneous vitrification of in vivo and IVF embryos in the same cryotube using identifiable green fluorescent pr …
Further, IVF embryos had a lower survival rate after warming, but a similar rate to the in vivo embryos to full-term development. Thi …
Optimised CO2-containing medium for in vitro culture and transportation of mouse preimplantation embryos without CO2 incubator.
Kikuchi Y, Wakayama S, Ito D, Ooga M, Wakayama T. Kikuchi Y, et al. Among authors: wakayama t. PLoS One. 2021 Dec 23;16(12):e0260645. doi: 10.1371/journal.pone.0260645. eCollection 2021. PLoS One. 2021. PMID: 34941870 Free PMC article.
Using an Anaero pouch, which is a CO2 gas-generating agent, to increase the CO2 partial pressure of CZB medium to 4%-5%, 2-cell embryos were cultured to the blastocyst stage in a sealed tube without a CO2 incubator at 37C. Further, the developmental rate to blastocyst and full- …
Using an Anaero pouch, which is a CO2 gas-generating agent, to increase the CO2 partial pressure of CZB medium to 4%-5%, 2-cell embryos were …
Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station.
Wakayama S, Ito D, Kamada Y, Shimazu T, Suzuki T, Nagamatsu A, Araki R, Ishikawa T, Kamimura S, Hirose N, Kazama K, Yang L, Inoue R, Kikuchi Y, Hayashi E, Emura R, Watanabe R, Nagatomo H, Suzuki H, Yamamori T, Tada MN, Osada I, Umehara M, Sano H, Kasahara H, Higashibata A, Yano S, Abe M, Kishigami S, Kohda T, Ooga M, Wakayama T. Wakayama S, et al. Among authors: wakayama t. Sci Adv. 2021 Jun 11;7(24):eabg5554. doi: 10.1126/sciadv.abg5554. Print 2021 Jun. Sci Adv. 2021. PMID: 34117068 Free PMC article.
Space radiation may cause DNA damage to cells and concern for the inheritance of mutations in offspring after deep space exploration. However, there is no way to study the long-term effects of space radiation using biological materials. Here, we developed a method to evalu …
Space radiation may cause DNA damage to cells and concern for the inheritance of mutations in offspring after deep space exploration. Howeve …
Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†.
Kamimura S, Inoue K, Mizutani E, Kim JM, Inoue H, Ogonuki N, Miyamoto K, Ihashi S, Itami N, Wakayama T, Ito A, Nishino N, Yoshida M, Ogura A. Kamimura S, et al. Among authors: wakayama t. Biol Reprod. 2021 Aug 3;105(2):543-553. doi: 10.1093/biolre/ioab096. Biol Reprod. 2021. PMID: 33982061 Free PMC article.
Thus, inhibition of class I and/or IIa HDACs in SCNT-derived embryos is enough for significant improvements in full-term development. In mouse SCNT, the exposure of reconstructed oocytes to HDAC inhibitors is limited to 8-10 h because longer inhibition with class I inhibit …
Thus, inhibition of class I and/or IIa HDACs in SCNT-derived embryos is enough for significant improvements in full-term development. …
Impact of chronic vitamin A excess on sperm morphogenesis in mice.
Yokota S, Sekine N, Wakayama T, Oshio S. Yokota S, et al. Among authors: wakayama t. Andrology. 2021 Sep;9(5):1579-1592. doi: 10.1111/andr.13013. Epub 2021 Jun 29. Andrology. 2021. PMID: 33818007 Free article.
Consistently, sperm motility and head morphology were significantly impaired in the VAE mice. DISCUSSION AND CONCLUSION: Our findings suggest that long-term dietary intake of VAE was able to influence both pre- and post-meiotic spermatogenesis. As a result of testicular to …
Consistently, sperm motility and head morphology were significantly impaired in the VAE mice. DISCUSSION AND CONCLUSION: Our findings sugges …
Removal of remodeling/reprogramming factors from oocytes and the impact on the full-term development of cloned embryos.
Konno S, Wakayama S, Ito D, Kazama K, Hirose N, Ooga M, Wakayama T. Konno S, et al. Among authors: wakayama t. Development. 2020 Aug 7;147(15):dev190777. doi: 10.1242/dev.190777. Development. 2020. PMID: 32665239
The size of the metaphase II spindle reduced immediately, but did recover when transferred into fresh oocytes. Though affected, the full-term developmental potential of these RRF-reduced oocytes with MII-spindle shrinkage was not lost after fertilization. ...
The size of the metaphase II spindle reduced immediately, but did recover when transferred into fresh oocytes. Though affected, the full- …
72 results