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delta133p53alpha, a natural p53 isoform, contributes to conditional reprogramming and long-term proliferation of primary epithelial cells.
Mondal AM, Zhou H, Horikawa I, Suprynowicz FA, Li G, Dakic A, Rosenthal B, Ye L, Harris CC, Schlegel R, Liu X. Mondal AM, et al. Among authors: suprynowicz fa. Cell Death Dis. 2018 Jul 3;9(7):750. doi: 10.1038/s41419-018-0767-7. Cell Death Dis. 2018. PMID: 29970881 Free PMC article.
We previously developed the technique of conditional reprogramming (CR), which allows primary epithelial cells from fresh or cryopreserved specimens to be propagated long-term in vitro, while maintaining their genetic stability and differentiation potential. ...More signif …
We previously developed the technique of conditional reprogramming (CR), which allows primary epithelial cells from fresh or cryopreserved s …
Conditional cell reprogramming involves non-canonical β-catenin activation and mTOR-mediated inactivation of Akt.
Suprynowicz FA, Kamonjoh CM, Krawczyk E, Agarwal S, Wellstein A, Agboke FA, Choudhury S, Liu X, Schlegel R. Suprynowicz FA, et al. PLoS One. 2017 Jul 10;12(7):e0180897. doi: 10.1371/journal.pone.0180897. eCollection 2017. PLoS One. 2017. PMID: 28700668 Free PMC article.
The combination of irradiated fibroblast feeder cells and Rho kinase inhibitor, Y-267362, converts primary epithelial cells growing in vitro into an undifferentiated adult stem cell-like state that is characterized by long-term proliferation. This cell culture method also …
The combination of irradiated fibroblast feeder cells and Rho kinase inhibitor, Y-267362, converts primary epithelial cells growing in vitro …
Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens.
Liu X, Krawczyk E, Suprynowicz FA, Palechor-Ceron N, Yuan H, Dakic A, Simic V, Zheng YL, Sripadhan P, Chen C, Lu J, Hou TW, Choudhury S, Kallakury B, Tang DG, Darling T, Thangapazham R, Timofeeva O, Dritschilo A, Randell SH, Albanese C, Agarwal S, Schlegel R. Liu X, et al. Among authors: suprynowicz fa. Nat Protoc. 2017 Feb;12(2):439-451. doi: 10.1038/nprot.2016.174. Epub 2017 Jan 26. Nat Protoc. 2017. PMID: 28125105 Free PMC article.