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A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila.
Gao G, McMahon C, Chen J, Rong YS. Gao G, et al. Among authors: rong ys. Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13999-4004. doi: 10.1073/pnas.0805843105. Epub 2008 Sep 4. Proc Natl Acad Sci U S A. 2008. PMID: 18772376 Free PMC article.
We developed an efficient method to repeatedly target a single locus in Drosophila. In this method, which we term "site-specific integrase mediated repeated targeting" (SIRT), an attP attachment site for the phage phiC31 integrase is first targeted to the vicinity of the g …
We developed an efficient method to repeatedly target a single locus in Drosophila. In this method, which we term "site-specific inte …