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Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades").
Mangeot PE, Guiguettaz L, Sohier TJM, Ricci EP. Mangeot PE, et al. Among authors: ricci ep. J Vis Exp. 2021 Mar 31;(169). doi: 10.3791/62245. J Vis Exp. 2021. PMID: 33871447
Nevertheless, these vectors can suffer from drawbacks such as integration of the transgene in the target cell genome, a limited cargo capacity, and long-term expression of the Cas9 protein and guide RNA in target cells. ...
Nevertheless, these vectors can suffer from drawbacks such as integration of the transgene in the target cell genome, a limited cargo capaci …