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Adaptive light-sheet microscopy for long-term, high-resolution imaging in living organisms.
Royer LA, Lemon WC, Chhetri RK, Wan Y, Coleman M, Myers EW, Keller PJ. Royer LA, et al. Among authors: myers ew. Nat Biotechnol. 2016 Dec;34(12):1267-1278. doi: 10.1038/nbt.3708. Epub 2016 Oct 31. Nat Biotechnol. 2016. PMID: 27798562
Here we present the AutoPilot framework, an automated method for spatiotemporally adaptive imaging that integrates (i) a multi-view light-sheet microscope capable of digitally translating and rotating light-sheet and detection planes in three dimensions and (ii) a computational m …
Here we present the AutoPilot framework, an automated method for spatiotemporally adaptive imaging that integrates (i) a multi-view light-sh …
Segmentation of center brains and optic lobes in 3D confocal images of adult fruit fly brains.
Lam SC, Ruan Z, Zhao T, Long F, Jenett A, Simpson J, Myers EW, Peng H. Lam SC, et al. Among authors: myers ew. Methods. 2010 Feb;50(2):63-9. doi: 10.1016/j.ymeth.2009.08.004. Epub 2009 Aug 19. Methods. 2010. PMID: 19698789 Free PMC article.
To obtain the locations of these control points, we derive an energy function that includes an image energy term defined by local pixel intensities and two internal energy terms that constrain the curve's smoothness and length. ...
To obtain the locations of these control points, we derive an energy function that includes an image energy term defined by local pix …
Automatic image analysis for gene expression patterns of fly embryos.
Peng H, Long F, Zhou J, Leung G, Eisen MB, Myers EW. Peng H, et al. Among authors: myers ew. BMC Cell Biol. 2007 Jul 10;8 Suppl 1(Suppl 1):S7. doi: 10.1186/1471-2121-8-S1-S7. BMC Cell Biol. 2007. PMID: 17634097 Free PMC article.
Lastly, we trained a suite of binary-classifiers, one for each anatomical annotation term in a controlled vocabulary or ontology that operate on the wavelet feature representation. ...
Lastly, we trained a suite of binary-classifiers, one for each anatomical annotation term in a controlled vocabulary or ontology that …