Gene targeting in adult rhesus macaque fibroblasts.
Meehan DT, Zink MA, Mahlen M, Nelson M, Sanger WG, Mitalipov SM, Wolf DP, Ouellette MM, Norgren RB Jr.
Meehan DT, et al. Among authors: mitalipov sm.
BMC Biotechnol. 2008 Mar 26;8:31. doi: 10.1186/1472-6750-8-31.
BMC Biotechnol. 2008.
PMID: 18366794
Free PMC article.
RESULTS: A primary culture of adult male fibroblasts was transfected with hTERT to overcome senescence and allow long term in vitro manipulations. Successful gene targeting of the HPRT locus in rhesus macaques was achieved by electroporating S-phase synchronized cells with …
RESULTS: A primary culture of adult male fibroblasts was transfected with hTERT to overcome senescence and allow long term in vitro m …