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Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation.
Seman SG, Bicer S, Julian MW, Mitchell JR, Kramer PJ, Crouser ED, Locke LW. Seman SG, et al. Among authors: locke lw. Biochem Biophys Res Commun. 2024 Jun 25;714:149993. doi: 10.1016/j.bbrc.2024.149993. Epub 2024 Apr 23. Biochem Biophys Res Commun. 2024. PMID: 38663096 Free article.
However, a current limitation of this model is its dependence on freshly isolated PBMCs obtained from whole blood. While cryopreservation is a common method for long-term sample preservation, the biological effects of freezing and thawing PBMCs on granuloma formation remai …
However, a current limitation of this model is its dependence on freshly isolated PBMCs obtained from whole blood. While cryopreservation is …
Obesity accentuates circadian variability in breathing during sleep in mice but does not predispose to apnea.
Davis EM, Locke LW, McDowell AL, Strollo PJ, O'Donnell CP. Davis EM, et al. Among authors: locke lw. J Appl Physiol (1985). 2013 Aug 15;115(4):474-82. doi: 10.1152/japplphysiol.00330.2013. Epub 2013 May 30. J Appl Physiol (1985). 2013. PMID: 23722707 Free PMC article.
Given the comorbidities associated with obesity in humans, we developed techniques for long-term recording of diaphragmatic EMG activity and polysomnography in obese mice to assess breathing patterns during sleep and to determine the effect of obesity on apnea generation. …
Given the comorbidities associated with obesity in humans, we developed techniques for long-term recording of diaphragmatic EMG activ …