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S/MAR Element Facilitates Episomal Long-Term Persistence of Adeno-Associated Virus Vector Genomes in Proliferating Cells.
Hagedorn C, Schnödt-Fuchs M, Boehme P, Abdelrazik H, Lipps HJ, Büning H. Hagedorn C, et al. Among authors: lipps hj. Hum Gene Ther. 2017 Dec;28(12):1169-1179. doi: 10.1089/hum.2017.025. Epub 2017 Jun 29. Hum Gene Ther. 2017. PMID: 28665147
Since AAV vectors do not possess an integrase activity, application is restricted to terminally differentiated tissues if transgene expression is required long term. To overcome this limitation and to generate AAV vectors that persist episomally in dividing cells, AAV vect …
Since AAV vectors do not possess an integrase activity, application is restricted to terminally differentiated tissues if transgene expressi …
S/MAR sequence confers long-term mitotic stability on non-integrating lentiviral vector episomes without selection.
Verghese SC, Goloviznina NA, Skinner AM, Lipps HJ, Kurre P. Verghese SC, et al. Among authors: lipps hj. Nucleic Acids Res. 2014 Apr;42(7):e53. doi: 10.1093/nar/gku082. Epub 2014 Jan 27. Nucleic Acids Res. 2014. PMID: 24474068 Free PMC article.
Here we describe a novel lentiviral vector (LV) that incorporates human ss-interferon scaffold/matrix-associated region sequences to provide an origin of replication for long-term mitotic maintenance of the episomal LTR circles. The resulting 'anchoring' non-integrating le …
Here we describe a novel lentiviral vector (LV) that incorporates human ss-interferon scaffold/matrix-associated region sequences to provide …
Viral hybrid-vectors for delivery of autonomous replicons.
Zhang W, Hagedorn C, Schulz E, Lipps HJ, Ehrhardt A. Zhang W, et al. Among authors: lipps hj. Curr Gene Ther. 2014 Feb;14(1):10-23. doi: 10.2174/1566523213666131223130024. Curr Gene Ther. 2014. PMID: 24365145 Review.
Gene therapeutic approaches offer great opportunities to treat genetic diseases which require long-term effects after a single administration of a customized vector. ...Predominantly used vectors for maintenance of therapeutic DNA and long-term transgene expression …
Gene therapeutic approaches offer great opportunities to treat genetic diseases which require long-term effects after a single admini …
Genomic cis-acting Sequences Improve Expression and Establishment of a Nonviral Vector.
Hagedorn C, Antoniou MN, Lipps HJ. Hagedorn C, et al. Among authors: lipps hj. Mol Ther Nucleic Acids. 2013 Sep 3;2(8):e118. doi: 10.1038/mtna.2013.47. Mol Ther Nucleic Acids. 2013. PMID: 24002728 Free PMC article.
Here we inserted genomic cis-acting sequences into pEPI and examined their impact on transgene expression, long-term stability, and vector establishment. We demonstrated that a ubiquitous chromatin-opening element (UCOE) mediated enhanced transgene expression, while an ins …
Here we inserted genomic cis-acting sequences into pEPI and examined their impact on transgene expression, long-term stability, and v …
A Novel Adenoviral Hybrid-vector System Carrying a Plasmid Replicon for Safe and Efficient Cell and Gene Therapeutic Applications.
Voigtlander R, Haase R, Mück-Hausl M, Zhang W, Boehme P, Lipps HJ, Schulz E, Baiker A, Ehrhardt A. Voigtlander R, et al. Among authors: lipps hj. Mol Ther Nucleic Acids. 2013 Apr 2;2(4):e83. doi: 10.1038/mtna.2013.11. Mol Ther Nucleic Acids. 2013. PMID: 23549553 Free PMC article.
We found that the plasmid replicon can be released from adenoviral DNA in murine liver resulting in long-term transgene expression. In conclusion, we demonstrate the efficacy of our novel HCAdV-pEPito hybrid-vector system in vitro and in vivo.Molecular Therapy-Nucleic Acid …
We found that the plasmid replicon can be released from adenoviral DNA in murine liver resulting in long-term transgene expression. I …
Controlled removal of a nonviral episomal vector from transfected cells.
Rupprecht S, Hagedorn C, Seruggia D, Magnusson T, Wagner E, Ogris M, Lipps HJ. Rupprecht S, et al. Among authors: lipps hj. Gene. 2010 Oct 15;466(1-2):36-42. doi: 10.1016/j.gene.2010.07.001. Epub 2010 Jul 16. Gene. 2010. PMID: 20621169
An ideal vector to be used in gene therapy should allow long-term and regulated expression of the therapeutic sequence, but in many cases, it would be most desirable to remove all ectopic vector sequences from the cell once expression is no longer required. ...We construct …
An ideal vector to be used in gene therapy should allow long-term and regulated expression of the therapeutic sequence, but in many c …
pEPito: a significantly improved non-viral episomal expression vector for mammalian cells.
Haase R, Argyros O, Wong SP, Harbottle RP, Lipps HJ, Ogris M, Magnusson T, Vizoso Pinto MG, Haas J, Baiker A. Haase R, et al. Among authors: lipps hj. BMC Biotechnol. 2010 Mar 15;10:20. doi: 10.1186/1472-6750-10-20. BMC Biotechnol. 2010. PMID: 20230618 Free PMC article.
The original pEPI-1 vector contains two mammalian transcription units and a total of 305 CpG islands, which are located predominantly within the vector elements necessary for bacterial propagation and known to be counterproductive for persistent long-term transgene express …
The original pEPI-1 vector contains two mammalian transcription units and a total of 305 CpG islands, which are located predominantly within …
Episomal vectors for gene therapy.
Ehrhardt A, Haase R, Schepers A, Deutsch MJ, Lipps HJ, Baiker A. Ehrhardt A, et al. Among authors: lipps hj. Curr Gene Ther. 2008 Jun;8(3):147-61. doi: 10.2174/156652308784746440. Curr Gene Ther. 2008. PMID: 18537590 Review.
The main goal of gene therapy is to treat loss-of-function genetic disorders by delivering correcting therapeutic DNA sequences into the nucleus of a cell, allowing its long-term expression at physiologically relevant levels. Manifold different vector systems for the thera …
The main goal of gene therapy is to treat loss-of-function genetic disorders by delivering correcting therapeutic DNA sequences into the nuc …
Long-term suppression of hepatitis B virus replication by short hairpin RNA expression using the scaffold/matrix attachment region-based replicating vector system pEPI-1.
Jenke AC, Wilhelm AD, Orth V, Lipps HJ, Protzer U, Wirth S. Jenke AC, et al. Among authors: lipps hj. Antimicrob Agents Chemother. 2008 Jul;52(7):2355-9. doi: 10.1128/AAC.00067-08. Epub 2008 May 12. Antimicrob Agents Chemother. 2008. PMID: 18474581 Free PMC article.
Since the emergence of viral resistance of hepatitis B virus (HBV) during treatment is becoming an important issue even with newer drugs, there is a need for alternative treatment options such as, for example, RNA interference (RNAi) technology. While short-term suppressio …
Since the emergence of viral resistance of hepatitis B virus (HBV) during treatment is becoming an important issue even with newer drugs, th …
Designing nonviral vectors for efficient gene transfer and long-term gene expression.
Jackson DA, Juranek S, Lipps HJ. Jackson DA, et al. Among authors: lipps hj. Mol Ther. 2006 Nov;14(5):613-26. doi: 10.1016/j.ymthe.2006.03.026. Epub 2006 Jun 19. Mol Ther. 2006. PMID: 16784894 Free article. Review.
Although the genetic therapy of human diseases has been conceptually possible for many years we still lack a vector system that allows safe and reproducible genetic modification of eukaryotic cells and ensures faithful long-term expression of transgenes. There is increasin …
Although the genetic therapy of human diseases has been conceptually possible for many years we still lack a vector system that allows safe …
13 results