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Page 1
Maximizing the Value of Cancer Drug Screening in Multicellular Tumor Spheroid Cultures: A Case Study in Five Head and Neck Squamous Cell Carcinoma Cell Lines.
Kochanek SJ, Close DA, Camarco DP, Johnston PA. Kochanek SJ, et al. SLAS Discov. 2020 Apr;25(4):329-349. doi: 10.1177/2472555219896999. Epub 2020 Jan 25. SLAS Discov. 2020. PMID: 31983262 Free PMC article.
It is conceivable that the viable cells present in MCTS cultures after drug exposure arise from drug-resistant populations that could represent a source of drug failure and recurrence. Long-term monitoring of treated MCTS cultures could provide a strategy to determine whet …
It is conceivable that the viable cells present in MCTS cultures after drug exposure arise from drug-resistant populations that could repres …
Pancreatic transduction by helper-dependent adenoviral vectors via intraductal delivery.
Morró M, Teichenne J, Jimenez V, Kratzer R, Marletta S, Maggioni L, Mallol C, Ruberte J, Kochanek S, Bosch F, Ayuso E. Morró M, et al. Among authors: kochanek s. Hum Gene Ther. 2014 Sep;25(9):824-36. doi: 10.1089/hum.2013.182. Hum Gene Ther. 2014. PMID: 25046147 Free PMC article.
Helper-dependent adenoviral vectors (HDAds) are promising tools for gene therapy because of their large cloning capacity, high levels of transgene expression, and long-term persistence in immunocompetent animals. Nevertheless, the ability of HDAds to transduce the pancreas …
Helper-dependent adenoviral vectors (HDAds) are promising tools for gene therapy because of their large cloning capacity, high levels of tra …
Foamy virus-adenovirus hybrid vectors for gene therapy of the arthritides.
Weber C, Armbruster N, Scheller C, Kreppel F, Kochanek S, Rethwilm A, Steinert AF. Weber C, et al. Among authors: kochanek s. J Gene Med. 2013 Mar-Apr;15(3-4):155-67. doi: 10.1002/jgm.2705. J Gene Med. 2013. PMID: 23554302
CONCLUSIONS: Our results indicate that FAD vectors are capable of efficient in vivo gene transfer to synovium and merit further investigation as a means of providing efficient and long-term intra-articular transgene expression for treatment of the arthritides....
CONCLUSIONS: Our results indicate that FAD vectors are capable of efficient in vivo gene transfer to synovium and merit further investigatio …
Interleukin-12 inhibits liver-specific drug-inducible systems in vivo.
Reboredo M, Zabala M, Mauleon I, De Las Rivas J, Kreppel F, Kochanek S, Prieto J, Hernandez-Alcoceba R, Kramer MG. Reboredo M, et al. Among authors: kochanek s. Gene Ther. 2008 Feb;15(4):277-88. doi: 10.1038/sj.gt.3303073. Epub 2007 Nov 22. Gene Ther. 2008. PMID: 18033307 Free article.
We have analyzed the kinetics of interleukin-12 (IL-12) expression controlled by the doxycycline (Dox)- and the mifepristone (Mif)-dependent systems using two long-term expressing vectors directed to liver: a plasmid administered by hydrodynamic injection and a high-capaci …
We have analyzed the kinetics of interleukin-12 (IL-12) expression controlled by the doxycycline (Dox)- and the mifepristone (Mif)-dependent …
Comparison of high-capacity and first-generation adenoviral vector gene delivery to murine muscle in utero.
Bilbao R, Reay DP, Wu E, Zheng H, Biermann V, Kochanek S, Clemens PR. Bilbao R, et al. Among authors: kochanek s. Gene Ther. 2005 Jan;12(1):39-47. doi: 10.1038/sj.gt.3302392. Gene Ther. 2005. PMID: 15483668
In utero gene delivery could offer the advantage of treatment at an early stage for genetic disorders such as Duchenne muscular dystrophy (DMD) in which the inevitable process of muscle degeneration is already initiated at birth. Furthermore, treatment of fetal muscle with …
In utero gene delivery could offer the advantage of treatment at an early stage for genetic disorders such as Duchenne muscular dystrophy (D …
Adenoviral vectors for gene transfer and therapy.
Volpers C, Kochanek S. Volpers C, et al. Among authors: kochanek s. J Gene Med. 2004 Feb;6 Suppl 1:S164-71. doi: 10.1002/jgm.496. J Gene Med. 2004. PMID: 14978759 Review.
Recent advances in the development of adenoviral vectors have brought considerable progress on issues like target cell specificity and tropism modification, long-term expression of the transgene, as well as immunogenicity and toxicity in vivo, and have suggested that the d …
Recent advances in the development of adenoviral vectors have brought considerable progress on issues like target cell specificity and tropi …
Long-term transgene expression in proliferating cells mediated by episomally maintained high-capacity adenovirus vectors.
Kreppel F, Kochanek S. Kreppel F, et al. Among authors: kochanek s. J Virol. 2004 Jan;78(1):9-22. doi: 10.1128/jvi.78.1.9-22.2004. J Virol. 2004. PMID: 14671083 Free PMC article.
High-capacity "gutless" adenovirus vectors (HC-AdV) mediate long-term transgene expression in resting cells in vitro and in vivo because of low toxicity and immunogenicity. ...After further improvements, this HC-AdV system might be suitable for gene therapy applications re …
High-capacity "gutless" adenovirus vectors (HC-AdV) mediate long-term transgene expression in resting cells in vitro and in vivo beca …
Selective depletion or blockade of Kupffer cells leads to enhanced and prolonged hepatic transgene expression using high-capacity adenoviral vectors.
Schiedner G, Hertel S, Johnston M, Dries V, van Rooijen N, Kochanek S. Schiedner G, et al. Among authors: kochanek s. Mol Ther. 2003 Jan;7(1):35-43. doi: 10.1016/s1525-0016(02)00017-5. Mol Ther. 2003. PMID: 12573616 Free article.
Interestingly, in the majority of animals of both strains depletion of KCs was sufficient to prevent the generation of anti-hAAT antibodies, resulting in prolonged transgene expression. Thus, short-term and selective depletion of hepatic macrophages at the same time signif …
Interestingly, in the majority of animals of both strains depletion of KCs was sufficient to prevent the generation of anti-hAAT antibodies, …
Therapeutic factor VIII levels and negligible toxicity in mouse and dog models of hemophilia A following gene therapy with high-capacity adenoviral vectors.
Chuah MK, Schiedner G, Thorrez L, Brown B, Johnston M, Gillijns V, Hertel S, Van Rooijen N, Lillicrap D, Collen D, VandenDriessche T, Kochanek S. Chuah MK, et al. Among authors: kochanek s. Blood. 2003 Mar 1;101(5):1734-43. doi: 10.1182/blood-2002-03-0823. Epub 2002 Oct 24. Blood. 2003. PMID: 12406898 Free article.
Intravenous administration of these vectors into hemophilic FVIII-deficient immunodeficient SCID mice (FVIIIKO-SCID) at a dose of 5 x 10(9) infectious units (IU) resulted in efficient hepatic gene delivery and long-term expression of supraphysiologic FVIII levels (exceedin …
Intravenous administration of these vectors into hemophilic FVIII-deficient immunodeficient SCID mice (FVIIIKO-SCID) at a dose of 5 x 10(9) …
Long-term transgene expression in the RPE after gene transfer with a high-capacity adenoviral vector.
Kreppel F, Luther TT, Semkova I, Schraermeyer U, Kochanek S. Kreppel F, et al. Among authors: kochanek s. Invest Ophthalmol Vis Sci. 2002 Jun;43(6):1965-70. Invest Ophthalmol Vis Sci. 2002. PMID: 12037006
CONCLUSIONS: In contrast to previous results with earlier generation adenoviral vectors, subretinal injection of an HC-Ad vector in immunocompetent rats resulted in long-term transgene expression without evidence of adverse immune reactions or significant toxicity, probabl …
CONCLUSIONS: In contrast to previous results with earlier generation adenoviral vectors, subretinal injection of an HC-Ad vector in immunoco …
21 results