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Two-stage focus-hold system for rapid ultra-sensitive read-out of large-area biochips.
J Microsc. 2009 Jun;234(3):251-4. doi: 10.1111/j.1365-2818.2009.03165.x.
J Microsc. 2009.
PMID: 19493102
Free PMC article.
The limited focal depth of high-NA objectives together with the demand for single-molecule sensitivity challenges traditional focus-hold systems. Various long- and short-term effects disturb the often multiple hour-long data-acquisitioning process and cause blurred or unus …
The limited focal depth of high-NA objectives together with the demand for single-molecule sensitivity challenges traditional focus-hold sys …
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