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Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector.
Argyros O, Wong SP, Niceta M, Waddington SN, Howe SJ, Coutelle C, Miller AD, Harbottle RP. Argyros O, et al. Gene Ther. 2008 Dec;15(24):1593-605. doi: 10.1038/gt.2008.113. Epub 2008 Jul 17. Gene Ther. 2008. PMID: 18633447
Long-term expression is demonstrated by monitoring the longitudinal luciferase expression profile for up to 6 months by means of in situ bioluminescent imaging. ...We conclude that the combination of a mammalian, tissue-specific promoter with the S/MAR element is sufficien …
Long-term expression is demonstrated by monitoring the longitudinal luciferase expression profile for up to 6 months by means of in s …
Delivery and long-term expression of a 135 kb LDLR genomic DNA locus in vivo by hydrodynamic tail vein injection.
Hibbitt OC, Harbottle RP, Waddington SN, Bursill CA, Coutelle C, Channon KM, Wade-Martins R. Hibbitt OC, et al. J Gene Med. 2007 Jun;9(6):488-97. doi: 10.1002/jgm.1041. J Gene Med. 2007. PMID: 17471590
Finally, BAC-end PCR, RT-PCR and immunohistochemistry demonstrate plasmid retention and long-term expression (4 months) of human LDLR in transfected hepatocytes. CONCLUSION: This is the first demonstration of somatic delivery and long-term expression of a genomic DN …
Finally, BAC-end PCR, RT-PCR and immunohistochemistry demonstrate plasmid retention and long-term expression (4 months) of human LDLR …