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Ex vivo reprogramming of human hematopoietic stem cells is accompanied by increased transcripts of genes regulating metabolic integrity.
Exp Hematol. 2023 Jun;122:41-54. doi: 10.1016/j.exphem.2023.03.006. Epub 2023 Mar 30.
Exp Hematol. 2023.
PMID: 37001723
Free article.
We have previously reported that human CD34(+) hematopoietic stem and progenitor cells (HSPCs) can be efficiently reprogrammed ex vivo and that the number of phenotypic HSCs with long-term repopulation capacity is expanded in the presence of a combination of cytokines and …
We have previously reported that human CD34(+) hematopoietic stem and progenitor cells (HSPCs) can be efficiently reprogrammed ex vivo and t …
Evaluation of a clinical-grade, cryopreserved, ex vivo-expanded stem cell product from cryopreserved primary umbilical cord blood demonstrates multilineage hematopoietic engraftment in mouse xenografts.
Schaniel C, Papa L, Meseck ML, Kintali M, Djedaini M, Zangui M, Iancu-Rubin C, Hoffman R.
Schaniel C, et al. Among authors: djedaini m.
Cytotherapy. 2021 Sep;23(9):841-851. doi: 10.1016/j.jcyt.2021.04.001. Epub 2021 May 19.
Cytotherapy. 2021.
PMID: 34023194
RESULTS: The authors found that the cryopreserved VPA-expanded grafts were characterized by a high degree of viability, retention of HSPC phenotypic subtypes and maintenance of long-term multilineage repopulation capacity in immunocompromised mice. ...
RESULTS: The authors found that the cryopreserved VPA-expanded grafts were characterized by a high degree of viability, retention of HSPC ph …
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Limited Mitochondrial Activity Coupled With Strong Expression of CD34, CD90 and EPCR Determines the Functional Fitness of ex vivo Expanded Human Hematopoietic Stem Cells.
Papa L, Djedaini M, Martin TC, Zangui M, Beaumont KG, Sebra R, Parsons R, Schaniel C, Hoffman R.
Papa L, et al. Among authors: djedaini m.
Front Cell Dev Biol. 2020 Dec 15;8:592348. doi: 10.3389/fcell.2020.592348. eCollection 2020.
Front Cell Dev Biol. 2020.
PMID: 33384995
Free PMC article.
While exposure of HSCs to ex vivo cultures expands the number of phenotypically identifiable HSCs, it frequently alters the transcriptomic and metabolic profiles, therefore, compromising their long-term (LT) hematopoietic reconstitution capacity. Within the heterogeneous p …
While exposure of HSCs to ex vivo cultures expands the number of phenotypically identifiable HSCs, it frequently alters the transcriptomic a …
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Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid.
Papa L, Djedaini M, Kintali M, Schaniel C, Hoffman R.
Papa L, et al. Among authors: djedaini m.
Methods Mol Biol. 2021;2185:267-280. doi: 10.1007/978-1-0716-0810-4_16.
Methods Mol Biol. 2021.
PMID: 33165854
The method described here utilizes a deacetylase inhibitor, valproic acid (VPA), to rapidly expand to a high degree the numbers of functional HSCs and committed progenitors (HPCs). The expanded HSCs are capable of establishing both short-term and long-term multiline …
The method described here utilizes a deacetylase inhibitor, valproic acid (VPA), to rapidly expand to a high degree the numbers of functiona …
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Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid.
Papa L, Djedaini M, Hoffman R.
Papa L, et al. Among authors: djedaini m.
J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59532.
J Vis Exp. 2019.
PMID: 31033947
The protocol detailed here describes the culture conditions and methodology to rapidly isolate CD34(+) cells and expand to a high degree a pool of primitive HSCs. The expanded HSCs are capable of establishing both short-term and long-term engraftment and are able to …
The protocol detailed here describes the culture conditions and methodology to rapidly isolate CD34(+) cells and expand to a high degree a p …
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