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RNA delivery by extracellular vesicles in mammalian cells and its applications.
O'Brien K, Breyne K, Ughetto S, Laurent LC, Breakefield XO. O'Brien K, et al. Among authors: breakefield xo. Nat Rev Mol Cell Biol. 2020 Oct;21(10):585-606. doi: 10.1038/s41580-020-0251-y. Epub 2020 May 26. Nat Rev Mol Cell Biol. 2020. PMID: 32457507 Free PMC article. Review.
The term 'extracellular vesicles' refers to a heterogeneous population of vesicular bodies of cellular origin that derive either from the endosomal compartment (exosomes) or as a result of shedding from the plasma membrane (microvesicles, oncosomes and apoptotic bodies). . …
The term 'extracellular vesicles' refers to a heterogeneous population of vesicular bodies of cellular origin that derive either from …
Long-Term Therapeutic Efficacy of Intravenous AAV-Mediated Hamartin Replacement in Mouse Model of Tuberous Sclerosis Type 1.
Prabhakar S, Cheah PS, Zhang X, Zinter M, Gianatasio M, Hudry E, Bronson RT, Kwiatkowski DJ, Stemmer-Rachamimov A, Maguire CA, Sena-Esteves M, Tannous BA, Breakefield XO. Prabhakar S, et al. Among authors: breakefield xo. Mol Ther Methods Clin Dev. 2019 Aug 16;15:18-26. doi: 10.1016/j.omtm.2019.08.003. eCollection 2019 Dec 13. Mol Ther Methods Clin Dev. 2019. PMID: 31534984 Free PMC article.
In a stochastic mouse model of TSC1 brain lesions, complete loss of Tsc1 is achieved in homozygous Tsc1-floxed mice in a subpopulation of neural cells in the brain by intracerebroventricular (i.c.v.) injection at birth of an adeno-associated virus (AAV) vector encoding Cre …
In a stochastic mouse model of TSC1 brain lesions, complete loss of Tsc1 is achieved in homozygous Tsc1-floxed mice in a subpopulation of ne …
Potential Transfer of Polyglutamine and CAG-Repeat RNA in Extracellular Vesicles in Huntington's Disease: Background and Evaluation in Cell Culture.
Zhang X, Abels ER, Redzic JS, Margulis J, Finkbeiner S, Breakefield XO. Zhang X, et al. Among authors: breakefield xo. Cell Mol Neurobiol. 2016 Apr;36(3):459-70. doi: 10.1007/s10571-016-0350-7. Epub 2016 Mar 7. Cell Mol Neurobiol. 2016. PMID: 26951563 Free PMC article.
These findings support the potential of EVs to deliver toxic expanded trinucleotide repeat RNAs from one cell to another, but further work will be needed to evaluate potential EV and cell-type specificity of transfer and effects of long-term exposure. It seems likely that …
These findings support the potential of EVs to deliver toxic expanded trinucleotide repeat RNAs from one cell to another, but further work w …
Herpes simplex virus type 1 amplicons and their hybrid virus partners, EBV, AAV, and retrovirus.
Oehmig A, Fraefel C, Breakefield XO, Ackermann M. Oehmig A, et al. Among authors: breakefield xo. Curr Gene Ther. 2004 Dec;4(4):385-408. doi: 10.2174/1566523043346129. Curr Gene Ther. 2004. PMID: 15578989 Review.
In contrast, vectors based on Epstein-Barr virus, adeno-associated virus, or retroviruses can mediate long-term transgene expression, as vector DNA is retained by episomal replication or chromosomal integration. ...
In contrast, vectors based on Epstein-Barr virus, adeno-associated virus, or retroviruses can mediate long-term transgene expression, …
Dynamics of transgene expression in human glioblastoma cells mediated by herpes simplex virus/adeno-associated virus amplicon vectors.
Lam P, Hui KM, Wang Y, Allen PD, Louis DN, Yuan CJ, Breakefield XO. Lam P, et al. Among authors: breakefield xo. Hum Gene Ther. 2002 Dec 10;13(18):2147-59. doi: 10.1089/104303402320987842. Hum Gene Ther. 2002. PMID: 12542846
Taken together, these data support employing AAV ITRs, in the context of HSV-1 amplicon vectors, to enhance short-term levels of transgene expression....
Taken together, these data support employing AAV ITRs, in the context of HSV-1 amplicon vectors, to enhance short-term levels of tran …
HSV-1 amplicon peptide display vector.
Spear MA, Schuback D, Miyata K, Grandi P, Sun F, Yoo L, Nguyen A, Brandt CR, Breakefield XO. Spear MA, et al. Among authors: breakefield xo. J Virol Methods. 2003 Jan;107(1):71-9. doi: 10.1016/s0166-0934(02)00193-3. J Virol Methods. 2003. PMID: 12445940
As the amplicon plasmids also contain the neomycin resistance gene (neo(r)), long term vector producer cell lines were created using G418 selection. ...
As the amplicon plasmids also contain the neomycin resistance gene (neo(r)), long term vector producer cell lines were created using …
Herpes simplex virus type 1 amplicon vector-mediated gene transfer to muscle.
Wang Y, Mukherjee S, Fraefel C, Breakefield XO, Allen PD. Wang Y, et al. Among authors: breakefield xo. Hum Gene Ther. 2002 Jan 20;13(2):261-73. doi: 10.1089/10430340252769789. Hum Gene Ther. 2002. PMID: 11812282
Although transgene expression was attenuated over time, significant improvement in long-term transgene expression and persistence of vector DNA was achieved, when compared with the first generation of recombinant HSV-1 vectors. ...
Although transgene expression was attenuated over time, significant improvement in long-term transgene expression and persistence of …
HSV-1-based vectors for gene therapy of neurological diseases and brain tumors: part II. Vector systems and applications.
Jacobs A, Breakefield XO, Fraefel C. Jacobs A, et al. Among authors: breakefield xo. Neoplasia. 1999 Nov;1(5):402-16. doi: 10.1038/sj.neo.7900056. Neoplasia. 1999. PMID: 10933055 Free PMC article. Review.
Helper virus-free HSV/AAV hybrid amplicon vectors have great promise in mediating long-term gene expression in the PNS and CNS for the treatment of various neurodegenerative disorders or chronic pain. ...
Helper virus-free HSV/AAV hybrid amplicon vectors have great promise in mediating long-term gene expression in the PNS and CNS for th …
Helper virus-free herpes simplex virus type 1 amplicon vectors for granulocyte-macrophage colony-stimulating factor-enhanced vaccination therapy for experimental glioma.
Herrlinger U, Jacobs A, Quinones A, Woiciechowsky C, Sena-Esteves M, Rainov NG, Fraefel C, Breakefield XO. Herrlinger U, et al. Among authors: breakefield xo. Hum Gene Ther. 2000 Jul 1;11(10):1429-38. doi: 10.1089/10430340050057503. Hum Gene Ther. 2000. PMID: 10910140
Subcutaneous vaccination of C57BL/6 mice with 5 x 10(5) irradiated HSVGM-transduced GL261 cells 7 days prior to intracerebral implantation of 10(6) wild-type GL261 cells yielded 60% long-term survivors (>80 days), similar to the 50% long-term survivors obtained b …
Subcutaneous vaccination of C57BL/6 mice with 5 x 10(5) irradiated HSVGM-transduced GL261 cells 7 days prior to intracerebral implantation o …
Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons.
Sena-Esteves M, Saeki Y, Camp SM, Chiocca EA, Breakefield XO. Sena-Esteves M, et al. Among authors: breakefield xo. J Virol. 1999 Dec;73(12):10426-39. doi: 10.1128/JVI.73.12.10426-10439.1999. J Virol. 1999. PMID: 10559361 Free PMC article.
Infection of human and dog primary gliomas with this system resulted in the production of retrovirus vectors for more than 1 week and the long-term retention and increase in transgene activity over time in these cell populations. ...
Infection of human and dog primary gliomas with this system resulted in the production of retrovirus vectors for more than 1 week and the lo …
19 results