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Prolonged airway explant culture enables study of health, disease, and viral pathogenesis.
Lee-Ferris RE, Okuda K, Galiger JR, Schworer SA, Rogers TD, Dang H, Gilmore R, Edwards C, Nakano S, Cawley AM, Pickles RJ, Gallant SC, Crisci E, Rivier L, Hagood JS, O'Neal WK, Baric RS, Grubb BR, Boucher RC, Randell SH. Lee-Ferris RE, et al. Among authors: boucher rc. bioRxiv [Preprint]. 2024 Feb 5:2024.02.03.578756. doi: 10.1101/2024.02.03.578756. bioRxiv. 2024. PMID: 38370820 Free PMC article. Preprint.
Ex vivo tissue models could overcome in vitro limitations, but methods for long-term maintenance of ex vivo tissue has not been established. We describe methods to culture human large airway explants, small airway explants, and precision-cut lung slices for at least 14 day …
Ex vivo tissue models could overcome in vitro limitations, but methods for long-term maintenance of ex vivo tissue has not been estab …
Modeling insights into SARS-CoV-2 respiratory tract infections prior to immune protection.
Chen A, Wessler T, Daftari K, Hinton K, Boucher RC, Pickles R, Freeman R, Lai SK, Forest MG. Chen A, et al. Among authors: boucher rc. Biophys J. 2022 May 3;121(9):1619-1631. doi: 10.1016/j.bpj.2022.04.003. Epub 2022 Apr 2. Biophys J. 2022. PMID: 35378080 Free PMC article.
We apply this baseline model in the absence of immune protection to explore immediate, short-term outcomes from novel SARS-CoV-2 depositions onto the air-ASL interface. ...
We apply this baseline model in the absence of immune protection to explore immediate, short-term outcomes from novel SARS-CoV-2 depo …
Potentiator ivacaftor abrogates pharmacological correction of ΔF508 CFTR in cystic fibrosis.
Cholon DM, Quinney NL, Fulcher ML, Esther CR Jr, Das J, Dokholyan NV, Randell SH, Boucher RC, Gentzsch M. Cholon DM, et al. Among authors: boucher rc. Sci Transl Med. 2014 Jul 23;6(246):246ra96. doi: 10.1126/scitranslmed.3008680. Sci Transl Med. 2014. PMID: 25101886 Free PMC article.
These findings demonstrate that chronic treatment with CFTR potentiators and correctors may have unexpected effects that cannot be predicted from short-term studies. Combining these drugs to maximize rescue of deltaF508 CFTR may require changes in dosing and/or development …
These findings demonstrate that chronic treatment with CFTR potentiators and correctors may have unexpected effects that cannot be predicted …
The adult cystic fibrosis airway microbiota is stable over time and infection type, and highly resilient to antibiotic treatment of exacerbations.
Fodor AA, Klem ER, Gilpin DF, Elborn JS, Boucher RC, Tunney MM, Wolfgang MC. Fodor AA, et al. Among authors: boucher rc. PLoS One. 2012;7(9):e45001. doi: 10.1371/journal.pone.0045001. Epub 2012 Sep 26. PLoS One. 2012. PMID: 23049765 Free PMC article.
Taken together, these results indicate that the adult CF lung microbiome is largely stable through periods of exacerbation and antibiotic treatment and that short-term compositional changes in the airway microbiota do not account for CF pulmonary exacerbations....
Taken together, these results indicate that the adult CF lung microbiome is largely stable through periods of exacerbation and antibiotic tr …
Similarities between UDP-glucose and adenine nucleotide release in yeast: involvement of the secretory pathway.
Esther CR Jr, Sesma JI, Dohlman HG, Ault AD, Clas ML, Lazarowski ER, Boucher RC. Esther CR Jr, et al. Among authors: boucher rc. Biochemistry. 2008 Sep 2;47(35):9269-78. doi: 10.1021/bi800855k. Epub 2008 Aug 12. Biochemistry. 2008. PMID: 18693752 Free PMC article.
Indeed, total adenine nucleotide release, like UDP-glucose release, did not vary with glucose concentration over the short term. The genetic basis of UDP-glucose release was explored through analysis of deletion mutants, aided by development of a novel bioassay for UDP-glu …
Indeed, total adenine nucleotide release, like UDP-glucose release, did not vary with glucose concentration over the short term. The …
Rationale for hypertonic saline therapy for cystic fibrosis lung disease.
Tarran R, Donaldson S, Boucher RC. Tarran R, et al. Among authors: boucher rc. Semin Respir Crit Care Med. 2007 Jun;28(3):295-302. doi: 10.1055/s-2007-981650. Semin Respir Crit Care Med. 2007. PMID: 17562499 Review.
Rehydrating airway surfaces may reduce or prevent lung injury and damage. Short- and longer term studies have shown that inhalation of hypertonic saline is well tolerated and improves lung function, reduces exacerbations, and improves quality of life in CF patients. ...
Rehydrating airway surfaces may reduce or prevent lung injury and damage. Short- and longer term studies have shown that inhalation o …
Mucus clearance and lung function in cystic fibrosis with hypertonic saline.
Donaldson SH, Bennett WD, Zeman KL, Knowles MR, Tarran R, Boucher RC. Donaldson SH, et al. Among authors: boucher rc. N Engl J Med. 2006 Jan 19;354(3):241-50. doi: 10.1056/NEJMoa043891. N Engl J Med. 2006. PMID: 16421365 Free article. Clinical Trial.
Mucus clearance and lung function were measured during 14-day baseline and treatment periods. RESULTS: Long-term inhalation of hypertonic saline without pretreatment with amiloride (i.e., with placebo pretreatment) resulted in a sustained (> or =8 hours) increase in 1-h …
Mucus clearance and lung function were measured during 14-day baseline and treatment periods. RESULTS: Long-term inhalation of hypert …
Mucin granule intraluminal organization in living mucous/goblet cells. Roles of protein post-translational modifications and secretion.
Perez-Vilar J, Mabolo R, McVaugh CT, Bertozzi CR, Boucher RC. Perez-Vilar J, et al. Among authors: boucher rc. J Biol Chem. 2006 Feb 24;281(8):4844-55. doi: 10.1074/jbc.M510520200. Epub 2005 Dec 23. J Biol Chem. 2006. PMID: 16377632 Free article.
Using a green fluorescent protein-mucin fusion protein (SHGFP-MUC5AC/CK) as a FRAP (fluorescence recovery after photobleaching) probe, we have assessed in living mucous cells the relative importance of different protein post-translational modifications on the intragranular organi …
Using a green fluorescent protein-mucin fusion protein (SHGFP-MUC5AC/CK) as a FRAP (fluorescence recovery after photobleaching) probe, we ha …
Chronic airway infection/inflammation induces a Ca2+i-dependent hyperinflammatory response in human cystic fibrosis airway epithelia.
Ribeiro CM, Paradiso AM, Schwab U, Perez-Vilar J, Jones L, O'neal W, Boucher RC. Ribeiro CM, et al. Among authors: boucher rc. J Biol Chem. 2005 May 6;280(18):17798-806. doi: 10.1074/jbc.M410618200. Epub 2005 Mar 3. J Biol Chem. 2005. PMID: 15746099 Free article.
We found that DeltaF508 CF bronchial epithelia were hyperinflammatory as defined by an increased basal and mucosal BK-induced interleukin (IL)-8 secretion. However, the CF hyperinflammation expressed in short-term (6-11-day-old) primary cultures of DeltaF508 bronchial epit …
We found that DeltaF508 CF bronchial epithelia were hyperinflammatory as defined by an increased basal and mucosal BK-induced interleukin (I …
pH-dependent intraluminal organization of mucin granules in live human mucous/goblet cells.
Perez-Vilar J, Olsen JC, Chua M, Boucher RC. Perez-Vilar J, et al. Among authors: boucher rc. J Biol Chem. 2005 Apr 29;280(17):16868-81. doi: 10.1074/jbc.M413289200. Epub 2005 Feb 17. J Biol Chem. 2005. PMID: 15718243 Free article.
Inhibition of protein synthesis or disorganization of the Golgi complex did not result in diminished intragranular SHGFP-MUC5AC/CK signals, consistent with long-term storage of the fusion protein. However, SHGFP-MUC5AC/CK was rapidly discharged from the granules upon incub …
Inhibition of protein synthesis or disorganization of the Golgi complex did not result in diminished intragranular SHGFP-MUC5AC/CK signals, …
18 results