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Transplanting FVIII/ET3-secreting cells in fetal sheep increases FVIII levels long-term without inducing immunity or toxicity.
Rodriguez M, Trevisan B, Ramamurthy RM, George SK, Diaz J, Alexander J, Meares D, Schwahn DJ, Quilici DR, Figueroa J, Gautreaux M, Farland A, Atala A, Doering CB, Spencer HT, Porada CD, Almeida-Porada G. Rodriguez M, et al. Among authors: almeida porada g. Nat Commun. 2023 Jul 14;14(1):4206. doi: 10.1038/s41467-023-39986-1. Nat Commun. 2023. PMID: 37452013 Free PMC article.
Thus, these studies attest to the feasibility, immunologic advantage, and safety of treating hemophilia A prior to birth....
Thus, these studies attest to the feasibility, immunologic advantage, and safety of treating hemophilia A prior to birth....
Autologous bone marrow-derived MSCs engineered to express oFVIII-FLAG engraft in adult sheep and produce an effective increase in plasma FVIII levels.
Trevisan B, Rodriguez M, Medder H, Lankford S, Combs R, Owen J, Atala A, Porada CD, Almeida-Porada G. Trevisan B, et al. Among authors: almeida porada g. Front Immunol. 2022 Dec 2;13:1070476. doi: 10.3389/fimmu.2022.1070476. eCollection 2022. Front Immunol. 2022. PMID: 36532079 Free PMC article.
METHODS: We tested, in a highly translational large animal (sheep) model, whether the unique immunological and biological properties of autologous bone marrow (BM)-derived mesenchymal stromal cells (MSCs) could enable them to serve as cellular delivery vehicles to provide long- …
METHODS: We tested, in a highly translational large animal (sheep) model, whether the unique immunological and biological properties of auto …
Hemophilia A: an ideal disease to correct in utero.
Porada CD, Rodman C, Ignacio G, Atala A, Almeida-Porada G. Porada CD, et al. Among authors: almeida porada g. Front Pharmacol. 2014 Dec 11;5:276. doi: 10.3389/fphar.2014.00276. eCollection 2014. Front Pharmacol. 2014. PMID: 25566073 Free PMC article. Review.
There is thus a need for novel treatments, such as those using stem cells and/or gene therapy, which have the potential to mediate long-term correction or permanent cure following a single intervention. ...
There is thus a need for novel treatments, such as those using stem cells and/or gene therapy, which have the potential to mediate long-t …
Treatment of Hemophilia A in Utero and Postnatally using Sheep as a Model for Cell and Gene Delivery.
Porada CD, Almeida-Porada G. Porada CD, et al. Among authors: almeida porada g. J Genet Syndr Gene Ther. 2012 May 25;S1:011. doi: 10.4172/2157-7412.S1-011. J Genet Syndr Gene Ther. 2012. PMID: 23264887 Free PMC article.
There is thus a need for novel treatments that can promise long-term or permanent correction. In contrast to existing protein based therapeutics, gene therapy offers to provide a permanent cure following few, or even a single, treatment. ...
There is thus a need for novel treatments that can promise long-term or permanent correction. In contrast to existing protein based t …
Systematic delineation of optimal cytokine concentrations to expand hematopoietic stem/progenitor cells in co-culture with mesenchymal stem cells.
Andrade PZ, dos Santos F, Almeida-Porada G, da Silva CL, S Cabral JM. Andrade PZ, et al. Among authors: almeida porada g. Mol Biosyst. 2010 Jul;6(7):1207-15. doi: 10.1039/b922637k. Epub 2010 Apr 27. Mol Biosyst. 2010. PMID: 20424784
The optimal cytokine cocktail obtained for HSC-MSC co-cultures was composed by SCF, Flt-3L and TPO (60, 55 and 50 ng mL(-1), respectively), resulting in 33-fold expansion in TNC, 17-fold in CD34(+) cells, 3-fold in CD34(+)CD90(+) cells and 21-fold in CFU-MIX. More importantly, th …
The optimal cytokine cocktail obtained for HSC-MSC co-cultures was composed by SCF, Flt-3L and TPO (60, 55 and 50 ng mL(-1), respectively), …
In vivo generation of beta-cell-like cells from CD34(+) cells differentiated from human embryonic stem cells.
Goodrich AD, Ersek A, Varain NM, Groza D, Cenariu M, Thain DS, Almeida-Porada G, Porada CD, Zanjani ED. Goodrich AD, et al. Among authors: almeida porada g. Exp Hematol. 2010 Jun;38(6):516-525.e4. doi: 10.1016/j.exphem.2010.03.002. Epub 2010 Mar 12. Exp Hematol. 2010. PMID: 20227460 Free PMC article.
An average of 1.51 ng/mL human C-peptide was detected in serum from eight animals transplanted with differentiated cell populations and assayed up to 55 months posttransplantation. Transplantation of as few as 23,500 cells resulted in long-term sustainable beta-cell-like a …
An average of 1.51 ng/mL human C-peptide was detected in serum from eight animals transplanted with differentiated cell populations and assa …
A Stro-1(+) human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system.
Gonçalves R, Lobato da Silva C, Cabral JM, Zanjani ED, Almeida-Porada G. Gonçalves R, et al. Among authors: almeida porada g. Exp Hematol. 2006 Oct;34(10):1353-9. doi: 10.1016/j.exphem.2006.05.024. Exp Hematol. 2006. PMID: 16982328 Free article.
Furthermore, we compared the results obtained with MSC as a feeder layer to traditional allogeneic stromal layers grown in long-term bone marrow culture media (LT-ST). METHODS: Adult human bone marrow CD34(+)-enriched cells were cultured in serum-free medium for 2 to 3 wee …
Furthermore, we compared the results obtained with MSC as a feeder layer to traditional allogeneic stromal layers grown in long-term …
In vivo haematopoietic potential of human neural stem cells.
Almeida-Porada G, Crapnell K, Porada C, Benoit B, Nakauchi H, Quesenberry P, Zanjani ED. Almeida-Porada G, et al. Br J Haematol. 2005 Jul;130(2):276-83. doi: 10.1111/j.1365-2141.2005.05588.x. Br J Haematol. 2005. PMID: 16029457 Free article.
Transplantation of secondary recipients with human CD45(+)/HLA-DR(+) cells from the BM of NSC primary recipients at 14 and 16 months post-transplant demonstrated that long-term engrafting HSC were present in these animals. At 6 months post-transplant, both NSC- and HSC-tra …
Transplantation of secondary recipients with human CD45(+)/HLA-DR(+) cells from the BM of NSC primary recipients at 14 and 16 months post-tr …
Human haematopoietic stem cells that mediate long-term in vivo engraftment are not susceptible to infection by human cytomegalovirus.
Crapnell KB, Almeida-Porada G, Khaiboullina S, St Jeor SC, Zanjani ED. Crapnell KB, et al. Among authors: almeida porada g. Br J Haematol. 2004 Mar;124(5):676-84. doi: 10.1111/j.1365-2141.2004.04827.x. Br J Haematol. 2004. PMID: 14871256 Free article.
A human/sheep xenograft model was used to evaluate whether long-term engrafting haematopoietic stem cells (HSC) are susceptible to human cytomegalovirus (HCMV) infection. ...At 5 weeks post-transplant several organs were HLA-DQalpha- and HCMV-positive, confirming that HCMV …
A human/sheep xenograft model was used to evaluate whether long-term engrafting haematopoietic stem cells (HSC) are susceptible to hu …
The sheep model of in utero gene therapy.
Porada CD, Park P, Almeida-Porada G, Zanjani ED. Porada CD, et al. Among authors: almeida porada g. Fetal Diagn Ther. 2004 Jan-Feb;19(1):23-30. doi: 10.1159/000074255. Fetal Diagn Ther. 2004. PMID: 14646413 Review.
Performing GT in utero would allow early correction prior to disease onset and is thus one of the few therapeutic modalities that could promise the birth of a healthy infant. Several features of the developing fetus may circumvent obstacles that have thus far been observed …
Performing GT in utero would allow early correction prior to disease onset and is thus one of the few therapeutic modalities that could prom …
30 results