Effect of polyunsaturated fatty acids ω-3 on the induction of activity and expression of CYP1A1 and CYP1A2 genes in the liver of rats under the influence of indole-3-carbinol

Bull Exp Biol Med. 2014 Jan;156(3):327-31. doi: 10.1007/s10517-014-2341-3. Epub 2014 Jan 16.

Abstract

Supplementation of the ration with eicosapentaenoic and docosahexaenoic ω-3 polyunsaturated fatty acids (PUFA) in doses of 0.3 and 1 g/kg body weight for 4 weeks had no effect on ethoxyresorufin O-dealkylase (EROD) activity and expression of the CYP1A1 gene in male Wistar rats, but caused a dose-dependent increase in methoxyresorufin O-dealkylase (MROD) activity of CYP1A2 (by 28 and 73%, respectively) without significant changes in CYP1A2 mRNA expression. ω-3 PUFA had no effect on the indole-3-carbinol-induced (20 mg/kg body weight over the last 7 days of the experiment) EROD activity and expression of CYP1A1 mRNA. The indole-3-carbinol-induced MROD activity was shown to increase by 6.2 times in rats not receiving ω-3 PUFA and only by 3.9 and 2.7 times in animals receiving ω-3 PUFA. The indole-3-carbinol-induced expression of CYP1A2 mRNA slightly increased in animals receiving ω-3 PUFA. Our results suggest that the effect of ω-3 PUFA on the induced and basal activity of CYP1A2 is not related to modulation of CYP1A2 gene expression.

MeSH terms

  • Animals
  • Base Sequence
  • Cytochrome P-450 CYP1A1 / genetics*
  • Cytochrome P-450 CYP1A2 / genetics*
  • DNA Primers
  • Fatty Acids, Omega-3 / pharmacology*
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Indoles / pharmacology*
  • Liver / drug effects*
  • Liver / enzymology
  • Male
  • Microsomes, Liver / enzymology
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DNA Primers
  • Fatty Acids, Omega-3
  • Indoles
  • indole-3-carbinol
  • Cytochrome P-450 CYP1A1
  • Cytochrome P-450 CYP1A2