A 26plex autosomal STR assay to aid human identity testing*

J Forensic Sci. 2009 Sep;54(5):1008-15. doi: 10.1111/j.1556-4029.2009.01110.x. Epub 2009 Jul 15.

Abstract

A short tandem repeat multiplex assay has been successfully developed with 25 autosomal loci plus the sex-typing locus amelogenin for a total of 26 amplified products in a single reaction. Primers for the loci were designed so that all of the amplicons present were distributed from 65 base pairs (bp) to less than 400 bp within a five-dye chemistry design with the fifth dye reserved for the sizing standard. A multiplex design strategy was developed to overcome challenges encountered in creating this assay. The limits of the multiplex were tested, resulting in the successful amplification of a wide range of genomic DNA sample concentrations from 2 ng to as low as 100 pg with 30 cycles of PCR. The 26plex has the potential to benefit the forensic community for reference sample testing and complex relationship evaluation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amelogenin / genetics
  • DNA Fingerprinting / methods*
  • DNA Primers
  • Forensic Anthropology
  • Humans
  • Polymerase Chain Reaction / methods*
  • Software
  • Tandem Repeat Sequences*

Substances

  • Amelogenin
  • DNA Primers