Psychotropic drug tenoten activates mitogen-activated MAP/ERK kinase regulatory cascade controlling the neuroprotective effects

Abstract

For evaluation of the molecular mechanisms underlying the effects of tenoten, a preparation based on ultralow-dose antibodies to S100 proteins and intended for the therapy of anxious and depressive disorders, we studied its influence on mitogen-activated ERK kinase cascade in Helix lucorum subpharyngeal ganglion complex. Western blot analysis showed that incubation of the ganglion preparation with tenoten resulted in significant activation of mitogen-activated ERK kinases, which was reduced by PD98059 (blocker of upstream protein kinase). This attests to a specific effect of the drug on mitogen-activated ERK kinase cascade. It is hypothesized that the therapeutic efficiency of tenoten is realed to activation of mitogen-activated ERK kinase cascade.