Small molecule mitochondrial F1F0 ATPase hydrolase inhibitors as cardioprotective agents. Identification of 4-(N-arylimidazole)-substituted benzopyran derivatives as selective hydrolase inhibitors

J Med Chem. 2004 Feb 26;47(5):1081-4. doi: 10.1021/jm030291x.

Abstract

In this paper we show that 4-aryl-CH2-imidazole-substituted benzopyran compounds with 3S,4R-stereochemistry are cardioprotective by inhibiting the F1F0 mitochondrial ATP hydrolase. Compounds (e.g., 13) with 3R,4S-stereochemistry act as mitochondrial KATP openers. This resulted from an inversion of stereochemistry for the F1F0 mitochondrial ATP hydrolase vs mitochondrial KATP. Structure-activity relationships for the inhibition of mitochondrial ATP hydrolase are also delineated. It is not clear how 13 (3R,4S) can selectively inhibit the hydrolytic activity of the F1F0 mitochondrial enzyme without interfering with the synthase activity.

MeSH terms

  • Adenosine Triphosphate / biosynthesis
  • Adenosine Triphosphate / metabolism
  • Animals
  • Benzopyrans / chemical synthesis*
  • Benzopyrans / chemistry
  • Benzopyrans / pharmacology
  • Cardiotonic Agents / chemical synthesis*
  • Cardiotonic Agents / chemistry
  • Cardiotonic Agents / pharmacology
  • Cattle
  • Citrate (si)-Synthase / metabolism
  • Electron Transport Complex IV / metabolism
  • Hydrolysis
  • Imidazoles / chemical synthesis*
  • Imidazoles / chemistry
  • Imidazoles / pharmacology
  • In Vitro Techniques
  • Mitochondrial Proton-Translocating ATPases / antagonists & inhibitors*
  • Myocardial Contraction / drug effects
  • Rats
  • Stereoisomerism
  • Structure-Activity Relationship

Substances

  • Benzopyrans
  • Cardiotonic Agents
  • Imidazoles
  • Adenosine Triphosphate
  • Electron Transport Complex IV
  • Citrate (si)-Synthase
  • F1F0-ATP synthase
  • Mitochondrial Proton-Translocating ATPases