An improved method for STR analysis of bloodstained denim

Int J Legal Med. 1999;112(6):388-90. doi: 10.1007/s004140050020.

Abstract

Indigo dye is used to dye denim and other fabrics. It is now accepted that if this is co-extracted with the DNA, it may inhibit PCR amplification. A simple, improved method is described for the extraction of DNA from bloodstained denim for PCR amplification and short tandem repeat (STR) analysis. The DNA was extracted by constructing a blotting system using capillary action to draw a saline solution through the denim. The transferred material was collected onto nylon membranes and these were processed by chelex extraction. A variety of coloured denim substrates and other heavily dyed fabrics, including case work samples were used. In all cases the DNA was extracted, amplified and typed correctly.

Publication types

  • Comparative Study

MeSH terms

  • Alleles
  • Blood Stains*
  • DNA / genetics
  • DNA / isolation & purification*
  • Humans
  • Luminescent Measurements
  • Polymerase Chain Reaction / methods
  • Tandem Repeat Sequences*

Substances

  • DNA